Trophoblast differentiation: Difference between revisions
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{{TimeCourse | {{TimeCourse | ||
|TCOverview=During mouse development, the implanting blastocyst embryo is composed of the inner cell mass (ICM) and the outer trophectoderm (TE) layer. Permanent stem cell lines can be derived from these two lineages; embryonic stem (ES) cells from ICM [1] [2] and trophoblast stem (TS) cells from TE [3]. TS cells can differentiate into multiple cell types only of the trophoblast lineage, while ES cells can differentiated into all three germ layer cell types. TS cells grow in the presence of fibroblast growth factor (FGF) 4 and mouse embryonic fibroblast-conditioned medium (MEF-CM). Removal of FGF4 and MEF-CM from the TS cell culture medium causes them to differentiate in vitro into trophoblast giant cells and other trophoblast subtypes. We want to investigate how gene expression is controlled in the trophoblast lineage. To do this we conducted a timecourse analysis of gene expression profiles of TS cells after the removal of FGF4 and MEF-CM.<br><br>References:<br>[1] Evans MJ and Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981. 292(5819): p154-6.<br>[2] Martin GR. Martin, Proc Natl Acad Sci U S A. 1981. 78(12): p7634-8.<br>[3] Tanaka S. Promotion of trophoblast stem cell proliferation by FGF4. Science. 1998. 282(5396): p2072-5.<br> | |||
|TCQuality_control=List of published key marker genes for your time course, if available with references:<br>* Cdx2, Eomes, Esrrb, Fgfr2: Marker genes for undifferentiated TS cells [3]<br>* Prl3d1, Prl3b1, Prl2c2: Marker genes for trophoblast giant cells [5] [6]<br>* Tpbpa: The Tpbpa gene is expressed in ectoplacental cone cells starting between embryonic days (E) 7.5 and 8.5, and later in the spongiotrophoblast layer of the mature placenta [7]<br>* Ascl2: The Ascl2 gene is expressed in the ectoplacental cone, the chorion and their derivatives in the placenta [8]<br><br><html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/200px-Trophoblast_Fig1.png'></html><br>Figure 1: B1 TS cells day0 (undifferentiated; scale bar = 100 um)<br><html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/200px-Trophoblast_Fig2.png'></html><br>Figure 2: B1 TS cells day5-differentiation (scale bar = 100 um)<br><html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/200px-Trophoblast_Fig3.png'></html><br>Figure 3: R1AB TS cells day0 (undifferentiated; scale bar = 100 um)<br><html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/200px-Trophoblast_Fig4.png'></html><br>Figure 4: R1AB TS cells day5-differentiation (scale bar = 100 um)<br><html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/1000px-Mouse_trophoblast_stem_cell_line.png' onclick='javascript:window.open("https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/1000px-Mouse_trophoblast_stem_cell_line.png", "imgwindow", "width=1000,height=500");' style='width:700px;cursor:pointer'/></html><br>Figure 5: CAGE expression of marker genes in TPM.<br><br>References:<br>[3] Tanaka S. Promotion of trophoblast stem cell proliferation by FGF4. Science. 1998. 282(5396): p2072-5.<br>[5] Faria TN, Ogren L, Talamantes F, Linzer DI, Soares MJ. Localization of placental lactogen-I in trophoblast giant cells of the mouse placenta. Biol Reprod. 1991. 44(2), p327-31<br>[6] Hu D and Cross JC. Development and function of trophoblast giant cells in the rodent placenta. Int J Dev Biol. 2010. 54(2-3): p341-54<br>[7] Hu D and Cross JC. Ablation of Tpbpa-positive trophoblast precursors leads to defects in maternal spiral artery remodeling in the mouse placenta. Dev Biol. 2011. 358(1): p231-9<br>[8] Guillemot F, Nagy A, Auerbach A, Rossant J, Joyner AL. Essential role of Mash-2 in extraembryonic development. Nature 1994. 371 (6495): p.333-6<br> | |||
|TCSample_description=TS cell lines are maintained in an undifferentiated state in the presence of FGF4 and 70% MEF-CM, and are differentiated by removal of FGF4 and MEF-CM for 0, 1, 2, 3, 4, 5, or 6 days (total 7 points). We used following three TS cell lines: a wild-type female TS cell line “B1”, a wild-type male TS cell line “Rybp”, and an Ring1A-/- male TS cell line “R1AB” (Ring1A-/- mice are healthy and exhibit no apparent phenotype in placental tissues [4]) .<br><br>References:<br>[4] del Mar Lorente M, Marcos-Gutiérrez C, Pérez C, Schoorlemmer J, Ramírez A, Magin T, Vidal M. Loss- and gain-of-function mutations show a polycomb group function for Ring1A in mice. Development. 2000. 127(23): p5093-100.<br> | |||
|Time_Course= | |Time_Course= | ||
|category_treatment=differentiation | |||
|collaborators=Haruhiko Koseki | |collaborators=Haruhiko Koseki | ||
|description=mouse_trophoblast_stem_cell_line | |description=mouse_trophoblast_stem_cell_line | ||
|germ_layer=ectoderm/ throphoblast | |||
|libraryids=CNhs13481,CNhs13514,CNhs13515,CNhs13516,CNhs13517,CNhs13518,CNhs13482,CNhs13519,CNhs13520,CNhs13521,CNhs13522,CNhs13523,CNhs13524,CNhs13525,CNhs13526,CNhs13527,CNhs13528,CNhs13529,CNhs13635,CNhs13636,CNhs13530 | |libraryids=CNhs13481,CNhs13514,CNhs13515,CNhs13516,CNhs13517,CNhs13518,CNhs13482,CNhs13519,CNhs13520,CNhs13521,CNhs13522,CNhs13523,CNhs13524,CNhs13525,CNhs13526,CNhs13527,CNhs13528,CNhs13529,CNhs13635,CNhs13636,CNhs13530 | ||
|number_time_points=7 | |||
|page_name=mouse_trophoblast_SCl_line | |page_name=mouse_trophoblast_SCl_line | ||
|primary_cells=cell line | |||
|series=IN_VITRO DIFFERENTIATION SERIES | |series=IN_VITRO DIFFERENTIATION SERIES | ||
|species=Mouse (Mus musculus) | |species=Mouse (Mus musculus) | ||
|tet_config=http://fantom.gsc.riken.jp/5/tet/search/?filename=mm9.cage_peak_phase1and2combined_tpm_ann_decoded.osc.txt.gz&file=1&c=1&c=987&c=988&c=989&c=990&c=991&c=992&c=993&c=994&c=995&c=996&c=997&c=998&c=999&c=1000&c=1001&c=1002&c=1003&c=1004&c=1005&c=1006&c=1007 | |||
|time_points= | |||
|time_span=6 days | |||
|timepoint_design=staged in-vitro diff | |||
|tissue_cell_type=trophoblast | |||
|zenbu_config=http://fantom.gsc.riken.jp/zenbu/gLyphs/#config=YRFbSnqnCJSpHzAzhtp_xC | |zenbu_config=http://fantom.gsc.riken.jp/zenbu/gLyphs/#config=YRFbSnqnCJSpHzAzhtp_xC | ||
}} | }} |
Revision as of 18:06, 3 February 2015
Series: | IN_VITRO DIFFERENTIATION SERIES |
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Species: | Mouse (Mus musculus) |
Genomic View: | Zenbu |
Expression table: | FILE |
Link to TET: | [{{{tet_file}}} TET] |
Sample providers : | Haruhiko Koseki |
Germ layer: | ectoderm/ throphoblast |
Primary cells or cell line: | cell line |
Time span: | 6 days |
Number of time points: | 7 |
CollapseOverview |
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During mouse development, the implanting blastocyst embryo is composed of the inner cell mass (ICM) and the outer trophectoderm (TE) layer. Permanent stem cell lines can be derived from these two lineages; embryonic stem (ES) cells from ICM [1] [2] and trophoblast stem (TS) cells from TE [3]. TS cells can differentiate into multiple cell types only of the trophoblast lineage, while ES cells can differentiated into all three germ layer cell types. TS cells grow in the presence of fibroblast growth factor (FGF) 4 and mouse embryonic fibroblast-conditioned medium (MEF-CM). Removal of FGF4 and MEF-CM from the TS cell culture medium causes them to differentiate in vitro into trophoblast giant cells and other trophoblast subtypes. We want to investigate how gene expression is controlled in the trophoblast lineage. To do this we conducted a timecourse analysis of gene expression profiles of TS cells after the removal of FGF4 and MEF-CM. |
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ExpandQuality control |
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Profiled time course samples
Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples