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Protocols:HeliScopeCAGE

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We started HeliScope CAGE with manual operation (with multi(8)-channel pipet), and achieved also automated preparation of the CAGE libraries to scale up the number of profiles (96 RNAs can be treated at once).

HeliScopeCAGE library construction

  • Manual library preparation (standard): OP-HELISCOPE-CAGE-v3.12
  • Manual library preparation (low quantity): OP-HELISCOPE-CAGE-v5.0
  • Automated library preparation: OP-HELISCOPE-CAGE-v4.0
  • HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0
  • Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0
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