Protocols:RNA extraction

We have used various RNA extraction protocols in FANTOM project.

All the following, but RNA extraction by trizol and ethanol, which was modified in order to keep both small and long RNA in the prep step, are standard.

• OP-RNA-extraction-totalRNA-TRIzol-ethanol-v1.0
  • File:Trizol ethanol ver.pdf
• OP-RNA-extraction-totalRNA-TRIzol-isopropanol-v1.0
  • File:Trizol isopropanol ver1.pdf
• OP-RNA-extraction-totalRNA-miRNeasy_Mini-v1.0
  • File:MiRNEasy ver1.pdf
• OP-RNA-extraction-totalRNA-RiboPure_minelute_cleanup-v1.0
  • File:RNeasy MinElute Cleanup Handbook.pdf
• OP-RNA-extraction-totalRNA-ToTALLY-RNA-v1.0
  • File:Totally RNA ver1.pdf