Difference between revisions of "ES to neuron"
Revision as of 12:43, 3 February 2015
|Series:||IN_VITRO DIFFERENTIATION SERIES|
|Species:||Mouse (Mus musculus)|
|Link to TET:|
|Sample providers :||Ana Pombo|
|Primary cells or cell line:||primary cells|
|Time span:||4 days|
|Number of time points:||5|
The differentiation from Embryonic Stem (ES) cells to specialized cells is one of the most important fields of research in modern cell biology. Stem cell therapies promise cures for a plethora of complex diseases such as neurodegeneration. ES cells have the potential to differentiate into any of the 200 different cell types that make up higher organisms. A key challenge towards understanding the mechanisms by which the different programs of gene expression are established during differentiation is the development of stem cell therapies and differentiation protocols into specific cellular lineages. To this end, we are interested in understanding the mechanisms that control important developmental regulator genes in ES cells, and the dynamic changes that occur during early cell differentiation to neuronal lineages.
Cells were grown at 37°C in a 5% (v/v) CO2 incubator. Mouse ES cells (cell line ES-46C; ES cell line E14tg2a expressing GFP under Sox1  were grown in GMEM medium (Invitrogen, # 21710025), supplemented with 10% (v/v) fetal calf serum (FCS; PAA, # A15-151), 2 U/ml LIF (Millipore, # ESG1107), 0.1 mM β-mercaptoethanol (Invitrogen, # 31350-010), 2 mM L-glutamine (Invitrogen, # 25030-024), 1 mM sodium pyruvate (Invitrogen, # 11360039), 1% penicillin-streptomycin (Invitrogen # 15140122), 1% MEM Non-Essential Amino Acids (Invitrogen, # 11140035) on gelatin-coated (0.1% (v/v)) Nunc T25 flasks. The medium was changed every day and cells were split every other day.
Profiled time course samples
|14357-155I1||ES-46C embryonic stem cells, neuronal differentiation||day00||biol_rep1|
|14358-155I2||ES-46C embryonic stem cells, neuronal differentiation||day01||biol_rep1|
|14359-155I3||ES-46C embryonic stem cells, neuronal differentiation||day02||biol_rep1|
|14360-155I4||ES-46C embryonic stem cells, neuronal differentiation||day03||biol_rep1|
|14361-155I5||ES-46C embryonic stem cells, neuronal differentiation||day04||biol_rep1|
|14362-155I6||ES-46C embryonic stem cells, neuronal differentiation||day00||biol_rep2|
|14363-155I7||ES-46C embryonic stem cells, neuronal differentiation||day01||biol_rep2|
|14364-155I8||ES-46C embryonic stem cells, neuronal differentiation||day02||biol_rep2|
|14365-155I9||ES-46C embryonic stem cells, neuronal differentiation||day03||biol_rep2|
|14366-156A1||ES-46C embryonic stem cells, neuronal differentiation||day04||biol_rep2|