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Protocols:rRNAdust

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Revision as of 11:55, 30 August 2012 by Marina (talk | contribs) (Created page with "This step is required to remove reads corresponding to rRNA from Helicos CAGE datasets. Since the error rate of Helicos is high and includes many insertion / deletion errors...")
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This step is required to remove reads corresponding to rRNA from Helicos CAGE datasets.

Since the error rate of Helicos is high and includes many insertion / deletion errors, the only viable option was to match sequences against rRNA sequences (U13369.1) using a non-heuristic alignment algorithm. Due to the amount of data a SSE parallelized version of Myers bit-parallel algorithm was implemented.

All reads matching the reference rRNA sequences with up to 2 errors are discarded at this step.