Supplementary Figure 3A Phylogenetic tree of the known OATPs (see Supplementary Figure 3B for the abbreviations) and new members (RIKEN Seqid 7536, 15434 and 18937).  The tree was constructed by the neighbor-joining method using CLUSTAL W.  Bootstrap probabilities were calculated with 1000 samplings.  The probabilities more than 90% are presented.  Branch lengths are drawn to scale.


Supplementary Figure 3B. Alignment of the amino acid sequences of the known OATPs (h: human, m: mouse, r: rat, h_OATP, GenBank U21943; h_OATP8,  AJ251506; h_OATP-B, AB020687; h_OATP-E, AB031051; h_OATP-F,  AF260704; h_LST-1, AF060500; h_PGT, U70867; m_oatp1, AB031813; m_oatp2-c,  AY007379; m_oatp2-l AB031814; m_LST-1, AB037202; r_oatp1, L19031;  r_oatp2: U88036;  r_oatp3, AF041105; r_oatp5, AF053317; r_OATP-K1, D79981; r_OATP-K2, AB012662; r_PGT, SwissProt Q00910) and new members (RIKEN Seqid 7536, 15434 and 18937), which was constructed using CLUSTAL W and CLUSTAL X viewer. The 12 putative transmembrane segments (red bars I to XII) were assigned on the basis of hydrophobicity analysis and sequence similarity. The region of the motif we detected is presented as a blue bar.