Saos calcification: Difference between revisions
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|species=Human (Homo sapiens) | |species=Human (Homo sapiens) | ||
|zenbu_config=http://fantom.gsc.riken.jp/zenbu/gLyphs/#config=vTTHn9oFB15qI8a1fB-Id | |zenbu_config=http://fantom.gsc.riken.jp/zenbu/gLyphs/#config=vTTHn9oFB15qI8a1fB-Id | ||
|TCOverview=The formation of bone is a multi-stage process. It begins with mesenchymal cells committed to becoming cartilage. These cells condense into nodules and differentiate into chondrocytes which proliferate rapidly to form the template for the developing bone. They secrete a cartilege-specific extracellular matrix. blood vessels sinvade the cartilage structure. Mesenchymal precursors surrounding the cartilage cells then differentiate into osteoblasts which begin to form an extracelular matrix specific for bone. At the same time the chondrocytes within the osteoblast shell begin to die from apoptosis leaving a space that becomes the bone marrow. osteoblasts themselves become embedded into the bone matrix and differentiate further into osteocytes. Bone is constantly being remodelled by the action of osteoclasts, cells of the monocyte lineage that degrade the bone and osteoblasts on the surface which replace it with new bone. Thus the formation and maintenance of bone is a dynamic process. | |||
During the formation of bone, osteoblasts lay down a matrix of hydroxyapatite, a mineral containing calciumand phosphorous which makes up to 50% of the weight of bone. this process of mineralisation can be simulated in vitro in cell cultures treated with chemicals to induce calcification. Many cell lines, primarily derived from osteosarcomas, can be induced to mineralise in this way, as can primary vascular smooth muscle cells. It is important to understand the process of mineralisation because formation of the bony skeleton is critical to the proper functioning of the vertebrate organism and because ectopic mineralisation can occur in genetic and environmental disease. For example, calcification is a key finding in forms of arterial disease including atherosclerosis. | |||
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Revision as of 14:15, 11 November 2014
| Series: | IN_VITRO DIFFERENTIATION SERIES |
|---|---|
| Species: | Human (Homo sapiens) |
| Genomic View: | Zenbu |
| Expression table: | [{{{tet_config}}} FILE] |
| Link to TET: | [{{{tet_file}}} TET] |
| Sample providers : | Kim Summers |
| Germ layer: | {{{germ_layer}}} |
| Primary cells or cell line: | {{{primary_cells}}} |
| Time span: | {{{time_span}}} |
| Number of time points: | {{{number_time_points}}} |
| Overview |
|---|
|
The formation of bone is a multi-stage process. It begins with mesenchymal cells committed to becoming cartilage. These cells condense into nodules and differentiate into chondrocytes which proliferate rapidly to form the template for the developing bone. They secrete a cartilege-specific extracellular matrix. blood vessels sinvade the cartilage structure. Mesenchymal precursors surrounding the cartilage cells then differentiate into osteoblasts which begin to form an extracelular matrix specific for bone. At the same time the chondrocytes within the osteoblast shell begin to die from apoptosis leaving a space that becomes the bone marrow. osteoblasts themselves become embedded into the bone matrix and differentiate further into osteocytes. Bone is constantly being remodelled by the action of osteoclasts, cells of the monocyte lineage that degrade the bone and osteoblasts on the surface which replace it with new bone. Thus the formation and maintenance of bone is a dynamic process. During the formation of bone, osteoblasts lay down a matrix of hydroxyapatite, a mineral containing calciumand phosphorous which makes up to 50% of the weight of bone. this process of mineralisation can be simulated in vitro in cell cultures treated with chemicals to induce calcification. Many cell lines, primarily derived from osteosarcomas, can be induced to mineralise in this way, as can primary vascular smooth muscle cells. It is important to understand the process of mineralisation because formation of the bony skeleton is critical to the proper functioning of the vertebrate organism and because ectopic mineralisation can occur in genetic and environmental disease. For example, calcification is a key finding in forms of arterial disease including atherosclerosis. |
| Sample description |
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|
{{{TCSample_description}}} |
| Quality control |
|---|
|
{{{TCQuality_control}}} |
Profiled time course samples
Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples
| 12662-134I7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr00min | biol_rep1 (A1 T0) |
| 12663-134I8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr15min | biol_rep1 (A1 T1) |
| 12664-134I9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr30min | biol_rep1 (A1 T2) |
| 12665-135A1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr45min | biol_rep1 (A1 T3) |
| 12666-135A2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr00min | biol_rep1 (A1 T4) |
| 12667-135A3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr20min | biol_rep1 (A1 T5) |
| 12668-135A4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr40min | biol_rep1 (A1 T6) |
| 12669-135A5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr00min | biol_rep1 (A1 T7) |
| 12670-135A6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr30min | biol_rep1 (A1 T8) |
| 12671-135A7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 03hr | biol_rep1 (A1 T9) |
| 12672-135A8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 04hr | biol_rep1 (A1 T10) |
| 12673-135A9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 08hr | biol_rep1 (A1 T11) |
| 12674-135B1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 24hr | biol_rep1 (A1 T12) |
| 12675-135B2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day04 | biol_rep1 (A1 T13) |
| 12676-135B3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day07 | biol_rep1 (A1 T14) |
| 12677-135B4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day14 | biol_rep1 (A1 T15) |
| 12678-135B5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day21 | biol_rep1 (A1 T16) |
| 12679-135B6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day28 | biol_rep1 (A1 T17) |
| 12760-136B6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr00min | biol_rep2 (A2 T0) |
| 12761-136B7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr15min | biol_rep2 (A2 T1) |
| 12762-136B8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr30min | biol_rep2 (A2 T2) |
| 12763-136B9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr45min | biol_rep2 (A2 T3) |
| 12764-136C1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr00min | biol_rep2 (A2 T4) |
| 12765-136C2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr20min | biol_rep2 (A2 T5) |
| 12766-136C3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr40min | biol_rep2 (A2 T6) |
| 12767-136C4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr00min | biol_rep2 (A2 T7) |
| 12768-136C5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr30min | biol_rep2 (A2 T8) |
| 12769-136C6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 03hr | biol_rep2 (A2 T9) |
| 12770-136C7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 04hr | biol_rep2 (A2 T10) |
| 12771-136C8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 08hr | biol_rep2 (A2 T11) |
| 12772-136C9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 24hr | biol_rep2 (A2 T12) |
| 12773-136D1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day04 | biol_rep2 (A2 T13) |
| 12774-136D2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day07 | biol_rep2 (A2 T14) |
| 12775-136D3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day14 | biol_rep2 (A2 T15) |
| 12776-136D4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day21 | biol_rep2 (A2 T16) |
| 12777-136D5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day28 | biol_rep2 (A2 T17) |
| 12858-137D5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr00min | biol_rep3 (A3 T0) |
| 12859-137D6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr15min | biol_rep3 (A3 T1) |
| 12860-137D7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr30min | biol_rep3 (A3 T2) |
| 12861-137D8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 00hr45min | biol_rep3 (A3 T3) |
| 12862-137D9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr00min | biol_rep3 (A3 T4) |
| 12863-137E1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr20min | biol_rep3 (A3 T5) |
| 12864-137E2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 01hr40min | biol_rep3 (A3 T6) |
| 12865-137E3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr00min | biol_rep3 (A3 T7) |
| 12866-137E4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 02hr30min | biol_rep3 (A3 T8) |
| 12867-137E5 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 03hr | biol_rep3 (A3 T9) |
| 12868-137E6 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 04hr | biol_rep3 (A3 T10) |
| 12869-137E7 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 08hr | biol_rep3 (A3 T11) |
| 12870-137E8 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | 24hr | biol_rep3 (A3 T12) |
| 12871-137E9 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day04 | biol_rep3 (A3 T13) |
| 12872-137F1 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day07 | biol_rep3 (A3 T14) |
| 12873-137F2 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day14 | biol_rep3 (A3 T15) |
| 12874-137F3 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day21 | biol_rep3 (A3 T16) |
| 12875-137F4 | Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification | day28 | biol_rep3 (A3 T17) |
