Protocols:Low quantity HeliScopeCAGE
From FANTOM5_SSTAR
We started HeliScope CAGE with manual operation (with multi(8)-channel pipet), and achieved also automated preparation of the CAGE libraries to scale up the number of profiles (96 RNAs can be treated at once).
HeliScopeCAGE library construction
- Manual library preparation (low quantity): OP-HELISCOPE-CAGE-v5.0
- HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0
- Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0
