Personal tools



Jump to: navigation, search

CAGEscan libraries are prepared using the nanoCAGE protocol OP-SOLEXA-nanoCAGE-Direct-v1.4, and sequenced paired-end according to standard procedures on Illumina Genome Analyzer IIx OP-Illumina-sequencing-PairedEnd-v3.0.

Post processing steps include:

  • rRNA dust on ribosomal DNA repeating unit
  • alignment of the CAGE reads with the genome
  • 5'-end based aggregation of CAGE tags

These steps above are same as the ones for the HeliScope protocol.