Protocols:nanoCAGEscan: Difference between revisions
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CAGEscan libraries are prepared using the nanoCAGE protocol | CAGEscan libraries are prepared using the nanoCAGE protocol OP-SOLEXA-nanoCAGE-Direct-v1.4, and sequenced paired-end according to standard procedures on Illumina Genome Analyzer IIx OP-Illumina-sequencing-PairedEnd-v3.0. | ||
Post processing steps include: | Post processing steps include: | ||
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*alignment of the CAGE reads with the genome | *alignment of the CAGE reads with the genome | ||
*5'-end based aggregation of CAGE tags | *5'-end based aggregation of CAGE tags | ||
These steps above are same as the ones for the HeliScope protocol. | |||
Revision as of 15:53, 30 August 2012
CAGEscan libraries are prepared using the nanoCAGE protocol OP-SOLEXA-nanoCAGE-Direct-v1.4, and sequenced paired-end according to standard procedures on Illumina Genome Analyzer IIx OP-Illumina-sequencing-PairedEnd-v3.0.
Post processing steps include:
- rRNA dust on ribosomal DNA repeating unit
- alignment of the CAGE reads with the genome
- 5'-end based aggregation of CAGE tags
These steps above are same as the ones for the HeliScope protocol.
