Introduction to FANTOM6
FANTOM (Functional ANnoTation Of the Mammalian genome) is a worldwide collaborative project aiming at identifying all functional elements in mammalian genomes. The goal of the sixth edition of the FANTOM project (FANTOM6) is to systematically elucidate the function of long non-coding RNAs (lncRNAs) in the human genome.
We envision the following as the main experimental strategy for FANTOM6:
- We are generating a reference set of genome-wide profiles to establish the basal state of the transcriptome and epigenome in each cell type.
- We are perturbing a set of lncRNAs in each cell type, and evaluating the molecular phenotype of the perturbation by CAGE profiling followed by bioinformatics analysis. The lncRNAs selected for perturbation are based on published transcripts as well as yet unpublished RNAseq assemblies specific to the cell types in the FANTOM5 collection.
- A selected subset of lncRNAs will be functionally characterized in more detail using other complementary technologies.
A preprint of our initial analysis of the function of lncRNAs is out:
Ramilowski J et al.: Functional Annotation of Human Long Non-Coding RNAs via Molecular Phenotyping, bioRxiv, 700864 (2019). doi: 10.1101/700864
Availability of data
The raw, processed and analyzed data is available: