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{{Coexpression_clusters
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|full_id=C1576_Mast_CD4_Natural_CD19_Basophils_CD8_CD34
|id=C1576
|ontology_enrichment_celltype=CL:0000738!6.25e-44!140;CL:0000037!2.41e-37!172;CL:0000566!2.41e-37!172;CL:0000988!1.77e-35!182;CL:0002031!9.01e-35!124;CL:0002032!1.07e-33!165;CL:0000837!1.07e-33!165;CL:0002087!4.53e-33!119;CL:0000542!3.71e-27!53;CL:0000051!3.71e-27!53;CL:0000838!8.23e-27!52;CL:0002057!8.64e-14!42;CL:0000084!3.18e-13!25;CL:0000827!3.18e-13!25;CL:0000766!4.56e-13!76;CL:0000945!9.76e-13!24;CL:0000826!9.76e-13!24;CL:0000791!4.40e-12!18;CL:0000789!4.40e-12!18;CL:0002420!4.40e-12!18;CL:0002419!4.40e-12!18;CL:0000790!4.40e-12!18;CL:0000860!1.97e-11!45;CL:0000763!5.21e-11!112;CL:0000049!5.21e-11!112;CL:0000557!5.40e-11!71;CL:0000839!4.03e-10!70;CL:0000236!4.31e-09!14;CL:0002009!1.20e-08!65;CL:0000625!5.30e-08!11;CL:0002194!5.44e-08!63;CL:0000576!5.44e-08!63;CL:0000040!5.44e-08!63;CL:0000559!5.44e-08!63
|ontology_enrichment_disease=
|ontology_enrichment_uberon=UBERON:0002390!1.15e-17!102;UBERON:0003061!1.15e-17!102;UBERON:0002193!7.75e-16!112;UBERON:0002371!1.35e-09!80;UBERON:0001474!2.11e-08!86;UBERON:0000178!3.52e-08!15;UBERON:0000179!3.52e-08!15;UBERON:0000463!3.52e-08!15;UBERON:0002405!4.13e-08!115;UBERON:0007023!2.96e-07!115
|tf_chipseq_enrich=BCL11A#53335;2:11.3477820414428:0.0115705693374132:0.0422819695951748!BCL3#602;3:20.728261682243:0.000232000521768923:0.00254997921416802!BDP1#55814;1:210.030681818182:0.0047522014418081:0.0223354109347462!BRF2#55290;1:214.91511627907:0.00464439788813553:0.0219244382136588!EBF1#1879;3:5.3438801079414:0.0118761556386811:0.0432262430734028!GATA2#2624;3:7.6469590401324:0.00427906187925213:0.0203256898898082!NFKB1#4790;4:4.39045073935507:0.00470773269252496:0.0221943255845535!TAF1#6872;5:3.34304628574529:0.00239460009087031:0.0135095958778308
|tfbs_overrepresentation=0.23317,0.0958562,0.877965,0.078254,0.310241,0.43203,0.0706464,0.5903,0.0539364,0.0424746,0.221396,0.346629,0.294377,0.201017,0.235304,0.101024,0.223746,0.158251,0.0865245,0.173528,0.29418,0.211047,0.13286,0.153106,0.0683222,0.332688,0.324018,0.146582,0.227906,0.150721,0.31768,0.182756,0.0298713,0.322378,0.108398,0.136785,0.578129,0.070583,0.192426,0.108683,0.155571,0.0406818,0.222325,0.287476,0.193292,0.0596699,0.0625547,0.0447422,0.178503,0.193726,0.243359,0.0886583,0.114185,0.150136,0.308837,0.0632035,0.172598,0.328738,0.127101,0.169687,0.0485674,0.236408,0.174369,0.123348,0.0892839,0.0827236,0.515105,0.0980005,0.139298,0.262613,0.547889,0.110232,0.143944,0.190944,0.012429,0.0283664,0.155593,0.0717582,0.0613084,0.0120583,0.154245,0.348799,0.0698251,0.183986,0.436695,0.38341,0.104918,0.323505,0.46357,0.102375,0.138802,0.0812726,0.153861,0.0777027,0.138224,0.184264,0.769004,0.242703,0.149747,0.413729,0.0939617,0.416542,0.225088,0.0610111,0.337778,0.0672793,0.109136,0.497282,0.228612,0.407248,0.0452737,0.0560173,0.23661,0.0293017,0.162943,0.0556189,0.0336673,0.0926302,0.220791,0.13579,0.262107,0.331577,0.222452,0.138843,0.164723,0.277102,0.146529,0.0245339,0.136609,0.214614,0.0973103,0.121587,0.0935301,0.0376876,0.200735,0.173595,0.105753,0.0905147,0.0544577,0.0065834,0.037671,0.203496,0.316207,0.246229,0.779726,0.112838,0.159217,0.1364,0.0640569,0.193163,0.311509,0.227801,0.282656,0.0439093,0.070163,0.188135,0.121467,0.350865,0.0846706,0.0555742,0.349932,0.153706,0.186743,0.23397,0.412853,0.183121,0.065826,0.203135,0.0663482,0.129698,0.281162,0.218835,0.278686,0.0885874,0.129639,0.145577,0.22277,0.284267,0.27935,0.0586816,0.250461,0.195671,0.0452583,0.185103,0.11983,0.190123,0.286036,0.0660586,0.134265,0.272191,0.172119,0.292239,0.0206847,0.351465,0.18064,0.0772176,0.126988,0.0984435,0.110187,0.176022,0.144361,0.0986228,0.0508565,0.0989516,0.21691,0.393782,0.412989,0.240045,0.319604,0.279218,0.0752697,0.167805,0.193654,0.180992,0.130092,0.265567,0.0367027,0.162511,0.102771,0.165687,0.225173,0.540012,0.246322,0.253699,0.120044,0.201982,0.220536,0.139142,0.0987108,0.09782,0.410084,0.554981,0.0687568,0.124225,0.1547,0.150448,0.0774663,0.398789,0.257365,0.331711,0.285222,0.511,0.158559,0.134615,0.186192,0.217494,0.132125,0.179065,0.540443,0.187334,0.250769,0.289141,0.289982,0.157983,0.158078,0.251693,0.172268,0.313249,0.113261,0.766691,0.101058,0.274448,0.25718,0.204241,0.0568578,0.131428,0.131579,0.148683,0.188945,0.122666,0.419402,0.103245,0.387442,0.627158,0.14011,0.0626022,0.2074,0.430549,0.875285,0.921608,0.814323,0.401202,0.944588,0.75925,0.493893,0.808889,0.396701,0.626645,0.541514,0.460217,0.675971,0.487888,0.205616,0.486439,0.720623,0.796904,0.410803,0.43779,0.579996,0.569955,0.766049,0.447317,0.292173,0.528152,0.284119,0.601223,0.176507,0.488132,0.336705,0.604733,0.545464,0.645432,0.518524,0.714193,0.706516,0.537972,0.591562,0.668584,0.385794,0.417358,0.450485,0.665656,0.470142,0.344006,0.688397,0.785741,0.426591,0.467379,0.420732,0.437202,0.628658,0.761311,0.592924,0.438692,0.660225,0.619033,0.541742,0.762222,0.790857,0.285074,0.605239,0.943261,0.668275,0.415372,0.362164,0.289863,0.507647,0.721501,0.409489,0.80581,0.688223,0.699429,0.707862,0.379008,0.423719,0.655494,0.256985,0.109234,0.11883,0.288602,0.779806,0.69124,0.766165,0.588284,0.284078,0.732646,0.737273,0.628596,0.337121,0.464864,0.588557,0.0740433,0.121457,0.351733,0.826323,0.773103,0.687274,0.441154,0.503865,0.660832,0.43388,0.483295,0.852766,0.103509,0.759561,0.239606,0.350461,0.495024,0.307173,0.246694,0.752605,0.598317,0.666615,0.597346,0.702113,0.632906,0.493489,0.229501,0.230597,0.195392,0.544572,0.627794,0.641833,0.383956,0.241286,0.0764074,0.217887,0.393543,0.214395,0.158849,0.732038,0.439833,0.427746,0.504398,0.456725,0.455709,0.784142,0.688249,0.708682,0.427794,0.398041,0.524026,0.347879,0.215832,0.593219,0.529253,0.64728,0.677026,0.319495,0.711229,0.827435,0.616333,0.407113,0.152579,0.738012,0.423517,0.224914,0.268941,0.706243,0.251608,0.365107,0.539805,0.356278,0.295455,0.144104,0.0972855,0.710825,0.80526,0.508875,0.725283,0.512109,0.658411,0.578577,0.818493,0.493686,0.469232,0.113555,0.0910724,0.00579137,0.126587,0.0940929,0.22877,0.343849,0.315824,0.622796,0.621499,0.29873,0.179502,0.43406,0.0715061,0.452515,0.201779,0.214491,0.171725,0.412647,0.102448,0.191372,0.029672,0.453305,0.421335,0.0510705,0.0762646,0.266371,0.183582,0.168118,0.145381,0.140277,0.251925,0.172066,0.0720324,0.00647589,0.19738,0.205854,0.32541,0.301472,0.507714,0.0818982,0.134306,0.108669,0.284804,0.178494,0.338195,0.146979,0.0518509,0.210475,0.294266,0.0790025,0.310539,0.580279,0.203728,0.275452,0.288155,0.265827,0.141761,0.153627,0.0186891,0.342497,0.171961,0.415228,0.00800917,0.272406,0.223952,0.327153,0.0496113,0.20949,0.0730994,0.0857377,0.323267,0.24204,0.129414,0.157037,0.252398,0.197279,0.146467,0.0912686,0.425114,0.342921,0.218241,0.0242146,0.10708,0.327369,0.339826,0.12998,0.123097,0.254847,0.300809,0.0898207,0.126545,0.0918749,0.0686325,0.0569043,0.0639461,0.387306,0.267408,0.312064,0.080156,0.274185,0.53753,0.0545112,0.510703,0.33844,0.0649137,0.0974216,0.595767,0.195675,0.174786,0.176557,0.396547,0.0472148,0.383224,0.229138,0.0639045,0.141461,0.0900204,0.228387,0.385962,0.334921,0.296227,0.208788,0.203834,0.0757402,0.430936,0.123626,0.270619,0.132705,0.285556,0.493429,0.233835,0.224471,0.262929,0.223204,0.143824,0.460679,0.431098,0.0775891,0.153079,0.62577,0.211515,0.125701,0.175283,0.204954,0.209341,0.576888,0.261552,0.084895,0.160115,0.0819954,0.10091,0.122658,0.151161,0.228398,0.056055,0.120552,0.41771,0.893893,0.529713,0.335207,0.434016,0.190688,0.0502346,0.278645,0.733648,0.0940982,0.0780533,0.564811,0.0993772,0.274769,0.448923,0.11602,0.344528,0.436413,0.0585992,0.20045,0.274438,0.911136,0.672453,0.319826,0.336512,0.173868,0.109708,0.174594,0.0809806,0.154589,0.318794,0.122767,0.0749261,0.176858,0.114361,0.347759,0.242462,0.0620267,0.211111,0.0738743,0.0882327,0.183058,0.150073,0.270522,0.16872,0.341388,0.0934526,0.359135,0.123404,0.430769,0.240825,0.3033,0.336623,0.163542,0.483244,0.27134,0.500871,0.239667,0.10226,0.29809,0.235147,0.32673,0.245965,0.116923,0.148426,0.0983658,0.228743,0.238223,0.156976,0.244789,0.267391,0.301441,0.21807,0.350528,0.382904,0.432408,0.190404,0.135865,0.145815,0.18137,0.180609,0.291283,0.273972,0.153023,0.400137,0.0834173,0.22307,0.160699,0.391307,0.166736,0.181253,0.0422294,0.263877,0.146486,0.431348,0.262486,0.294204,0.131936,0.288826,0.246621,0.463617,0.119485,0.0965376,0.377203,0.279263,0.336564,0.35463,0.581771,0.800557,0.0594155,0.222245,0.208795,0.0713793,0.212621,0.594469,0.53612,0.629713,0.581874,0.355154,0.145569,0.619037,0.537088,0.603804,0.629445,0.306963,0.569605,0.526495,0.215897,0.550495,0.764863,0.820986,0.610475,0.881697,0.561689,0.306829,0.685628,0.623136,0.349408,0.861766,0.855024,0.349068,0.70429,0.476117,0.557035,0.427789,0.689998,0.737642,0.886619,0.433702,0.258394,0.644878,0.432123,0.759415,0.661773,0.494975,0.28382,0.392155,0.626931,0.371758,0.457109,0.562417,0.890566,0.85279,0.254547,0.673352,0.821811,0.680843,0.746881,0.541378,0.47213,0.29416,0.922144,0.666904,0.914902,0.315485,0.311289,0.318577,0.4488,0.389888,0.603509,0.511254,0.193221,0.246387,0.224252,0.587237,0.155054,0.466978,0.0379309,0.257848,0.353942,0.192234,0.427276,0.400353,0.466967,0.283682,0.424668,0.462212,0.474058,0.218352,0.122953,0.477016,0.831202,0.498956,0.465544,0.55798,0.708394,0.751439,0.367723,0.34194,0.592696,0.226514,0.644904,0.380115,0.763919,0.317485,0.265627,0.220564,0.556368,0.262394,0.307462,0.135076,0.469588,0.664443,0.274593,0.490712,0.541363,0.709432,0.583627,0.531474,0.364391,0.562305,0.528646,0.361524,0.31558,0.412744,0.54408,0.259136,0.513592,0.415367,0.240393,0.45585,0.36864,0.181824,0.456862,0.453004,0.680686,0.557093,0.330982,0.382214,0.553161,0.639572,0.326875,0.339393,0.117434,0.306231,0.298354,0.646049,0.784543,0.457741,0.273855,0.426239,0.748909,0.656093,0.572661,0.412586,0.178696,0.41502,0.55475,0.66153,0.523749,0.499347,0.307426,0.790204,0.557732,0.490527,0.714189,0.44799,0.813041,0.318338,0.276338,0.126433,0.417412,0.318174,0.0555558,0.295666,0.0899804,0.293641,0.116969,0.401231,0.255983,0.297663,0.256708,0.338258,0.322052,0.145568,0.153833,0.261242,0.38389,0.54721,0.388459,0.321362,0.627637,0.280757,0.301366,0.340343,0.343265,0.501812,0.462092,0.336254,0.308589,0.291523,0.19697,0.309816,0.0537285,0.15063,0.198077,0.281455,0.348739,0.419895,0.138495,0.208834,0.32256,0.377566,0.342366,0.109684,0.322704,0.403482,0.181288,0.230841,0.352328,0.255358,0.351665,0.265112,0.0633313,0.10772,0.0854747,0.0747609,0.406458,0.312881,0.512825,0.244656,0.192188,0.158985,0.174153,0.0972854,0.333309,0.249919,0.339517,0.303699,0.198326,0.394084,0.126349,0.282501,0.252005,0.390891,0.238377,0.334983,0.197875,0.287909,0.289389,0.117618,0.392533,0.396962,0.316002,0.330317,0.321404,0.225079,0.300542,0.379651,0.185715,0.345505,0.375521,0.271405,0.311201,0.0266853,0.306427,0.367733,0.246925,0.208775,0.390438,0.0670191,0.270094,0.276922,0.156279,0.313647,0.461742,0.422872,0.336468,0.13327,0.313545,0.17149,0.0877361,0.0880065,0.238969,0.126615,0.0669467,0.0879453,0.474066,0.396935,0.326779,0.292869,0.358198,0.29552,0.131776,0.158116,0.104606,0.194814,0.104167,0.168186,0.108081,0.123829,0.205215,0.175247,0.0724304,0.109077,0.144842,0.0604892,0.214671,0.316879,0.442567,0.109896,0.508546,0.224871,0.398887,0.389061,0.310732,0.349611,0.445995,0.385709,0.278202,0.426488,0.400591,0.209809,0.253154,0.319519,0.309489,0.110045,0.362154,0.480785,0.185476,0.336314,0.344469,0.184651,0.303359,0.297596,0.42392,0.425912,0.380826,0.259046,0.345068,0.151046,0.483953,0.347171,0.321735,0.15463,0.13907,0.470121,0.269432,0.250466,0.409882,0.115686,0.408975,0.162633,0.342661,0.0420678,0.248127,0.33299,0.0858973,0.319112,0.526476
}}

Latest revision as of 11:47, 17 September 2013


Full id: C1576_Mast_CD4_Natural_CD19_Basophils_CD8_CD34



Phase1 CAGE Peaks

Hg19::chr10:32621291..32621298,-p@chr10:32621291..32621298
-
Hg19::chr14:50329808..50329834,+p@chr14:50329808..50329834
+
Hg19::chr1:144520117..144520134,+p@chr1:144520117..144520134
+
Hg19::chr4:40198691..40198704,+p2@RHOH
Hg19::chr5:109026781..109026805,-p@chr5:109026781..109026805
-


Enriched pathways on this co-expression cluster<b>Summary:</b><br>Canonical pathway gene sets were compiled from Reactome, Wikipathways and KEGG. For the major signaling pathways, the transcriptionally-regulated genes (downstream targets) were obtained from Netpath. Combined, the canonical pathways and downstream targets totaled 489 human gene sets. The corresponding M. musculus gene sets were inferred by homology using the HomoloGene database. Enrichment for each of the canonical 489 pathways and gene sets included in the co-expression cluster was assessed by the hypergeometric probability. The resulting P values were also then adjusted by the Benjamini-Hochberg method for multiple comparisons.<br><b>Analyst: </b>Emmanuel Dimont<br><br>link to source dataset<br>data


No results for this coexpression

Enriched Gene Ontology terms on this co-expression cluster<b>Summary:</b> Results for GOStat analysis on co-expressed clusters. Each cluster with promoters mapping to at least two different genes was analysed with GOStat (PMID: 14962934) with default parameter. <br><b>Analyst:</b> Erik Arner<br><br>link to source dataset<br>data


No GOStat results

Enriched sample ontology terms on this co-expression cluster<b>Summary:</b>To summarize promoter activities (expression profile of a TSS region) across ~1000 samples, we performed enrichment analysis based on FANTOM5 Sample Ontology (FF ontology). The question here is “in which type of samples the promoter is more active”. To answer this question, we compared expressions (TPMs) in the samples associated with a sample ontology term and the rest of the samples by using the Mann-Whitney rank sum test. To summarize ontologies enriched in this co-expression cluster, we ran the same analysis on an averaged expression profile of all promoters that make up. <b>Analyst:</b> Hideya Kawaji <br><br>links to source dataset<br><br>cell_data<br>uberon_data<br><br>


Cell Type
Ontology termp-valuen
leukocyte7.08e-51136
lymphoid lineage restricted progenitor cell8.35e-4752
lymphocyte1.40e-4553
common lymphoid progenitor1.40e-4553
nucleate cell3.22e-4555
hematopoietic lineage restricted progenitor cell2.13e-44120
nongranular leukocyte8.33e-43115
hematopoietic stem cell2.40e-40168
angioblastic mesenchymal cell2.40e-40168
hematopoietic cell5.82e-39177
hematopoietic oligopotent progenitor cell1.54e-35161
hematopoietic multipotent progenitor cell1.54e-35161
lymphocyte of B lineage1.28e-2324
pro-B cell1.28e-2324
mature alpha-beta T cell5.63e-2318
alpha-beta T cell5.63e-2318
immature T cell5.63e-2318
mature T cell5.63e-2318
immature alpha-beta T cell5.63e-2318
T cell4.62e-2025
pro-T cell4.62e-2025
B cell3.00e-1714
CD8-positive, alpha-beta T cell1.84e-1411
CD4-positive, alpha-beta T cell1.31e-096
myeloid leukocyte7.59e-0972
myeloid lineage restricted progenitor cell1.15e-0766
classical monocyte1.33e-0742
CD14-positive, CD16-negative classical monocyte1.33e-0742
granulocyte monocyte progenitor cell1.72e-0767
Uber Anatomy
Ontology termp-valuen
hematopoietic system4.78e-1598
blood island4.78e-1598
hemolymphoid system6.67e-14108
immune system9.62e-0993
blood2.72e-0815
haemolymphatic fluid2.72e-0815
organism substance2.72e-0815
bone marrow5.88e-0776


Overrepresented TFBS (DNA) motifs on this co-expression cluster<b>Summary:</b>The values shown are the p-values for overrepresentation of the motif in this coexpression cluster. So a small p-value means a strong overrepresentation. <b>Analyst:</b> Michiel de Hoon <br><br>link to source data <br> Novel motifs <br>data <br><br> Jaspar motifs <br>data


Novel motifs



JASPAR motifs

Motifs-log10(p-value)
MA0003.10.481059
MA0004.10.626788
MA0006.11.14863
MA0007.12.50943
MA0009.11.11255
MA0014.10.161847
MA0017.10.496101
MA0019.10.784036
MA0024.11.00439
MA0025.11.24931
MA0027.12.73598
MA0028.11.18667
MA0029.11.02437
MA0030.11.01252
MA0031.10.945567
MA0038.10.736268
MA0040.11.03042
MA0041.10.64667
MA0042.10.612397
MA0043.11.11288
MA0046.11.10134
MA0048.10.195817
MA0050.10.613347
MA0051.10.731787
MA0052.11.03442
MA0055.10.603517
MA0056.10
MA0057.10.189508
MA0058.10.523638
MA0059.11.29682
MA0060.10.321781
MA0061.11.44118
MA0063.10
MA0066.10.736721
MA0067.11.43651
MA0068.10.247962
MA0069.11.09737
MA0070.11.08584
MA0071.10.695094
MA0072.11.0812
MA0073.10.0469445
MA0074.10.730989
MA0076.11.33654
MA0077.11.07324
MA0078.10.836493
MA0081.10.522409
MA0083.11.12028
MA0084.11.62584
MA0087.11.07867
MA0088.10.132006
MA0089.10
MA0090.10.556637
MA0091.10.629594
MA0092.10.588959
MA0093.10.456342
MA0095.10
MA0098.10
MA0100.10.750974
MA0101.11.16366
MA0103.10.442769
MA0105.11.00065
MA0106.10.779931
MA0107.10.985964
MA0108.20.94238
MA0109.10
MA0111.10.5713
MA0113.11.8711
MA0114.10.973423
MA0115.11.35895
MA0116.10.382106
MA0117.11.1512
MA0119.10.504689
MA0122.11.17777
MA0124.11.31673
MA0125.11.23103
MA0130.10
MA0131.10.855546
MA0132.10
MA0133.10
MA0135.11.14377
MA0136.10.743749
MA0139.10.759606
MA0140.10.692258
MA0141.10.520334
MA0142.10.912455
MA0143.10.797437
MA0144.10.356507
MA0145.10.143698
MA0146.10.143081
MA0147.11.00307
MA0148.10.653474
MA0149.10.681935
MA0062.20.777733
MA0035.20.691506
MA0039.20.0575907
MA0138.20.837782
MA0002.20.30855
MA0137.20.47779
MA0104.21.54865
MA0047.20.767096
MA0112.20.136971
MA0065.20.146576
MA0150.10.550239
MA0151.10
MA0152.10.69926
MA0153.11.21379
MA0154.10.514087
MA0155.10.130813
MA0156.10.480289
MA0157.10.883854
MA0158.10
MA0159.10.386729
MA0160.10.669748
MA0161.10
MA0162.10.21472
MA0163.10.680296
MA0164.10.810347
MA0080.20.456437
MA0018.20.781393
MA0099.20.699641
MA0079.20.00732454
MA0102.21.66336
MA0258.10.345771
MA0259.11.02361
MA0442.10



ENCODE TF ChIP-seq peak enrichment analysis<b>Summary:</b> For each TF and each co-expression cluster, the number of promoters with ENCODE TF ChIP signal was compared with the rest of promoters from the robust set using Fisher's exact test. Clusters with significant ChIP enrichment (q <= 0.05) after Benjamini-Hochberg correction were retained. <br><b>Analyst:</b> Erik Arner<br><br>link to source dataset<br><br>data


(#promoters = Number of promoters in this coexpression cluster that have ChIP signal of the TF)

TF#promotersEnrichmentp-valueq-value
BCL11A#53335211.34778204144280.01157056933741320.0422819695951748
BCL3#602320.7282616822430.0002320005217689230.00254997921416802
BDP1#558141210.0306818181820.00475220144180810.0223354109347462
BRF2#552901214.915116279070.004644397888135530.0219244382136588
EBF1#187935.34388010794140.01187615563868110.0432262430734028
GATA2#262437.64695904013240.004279061879252130.0203256898898082
NFKB1#479044.390450739355070.004707732692524960.0221943255845535
TAF1#687253.343046285745290.002394600090870310.0135095958778308



Relative expression of the co-expression cluster<b>Summary:</b>Co-expression clusters are compared against FANTOM5 samples to obtain relative expression. <br><b>Analyst:</b>NA<br><br>link to data source<br> data


This analysis result is provided for C0 - C305 clusters.